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IL-6 gene expression is activated by the N protein which binds to the NF-kB regulatory element on IL-6 promoter and facilitates its translocation from cytosol to nucleus

doi: 10.1016/j.bbi.2017.02.007 32 PrandovszkyEGaskellEMartinHDubeyJPWebsterJPMcConkeyGA

N-terminally truncated isoforms (N) lack the transactivation domain and appear to act in a dominant-negative manner by either competing with the TA isoforms of p63 and p53 for DNA binding sites or by direct protein interaction

Then, mice were subjected to a glucose tolerance test (2 g glucose/kg b.w

Transient Transfection of Human GSTP1 Gene After plating on collagen-coated plates for 24 h, HepG2 cells were transiently transfected with 0.1 g/1 10 4 cells hGSTP1 expression plasmid (SC119655, Origene, Rockville, MD, United States) or accompanying empty pCMV6-XL5 vector (pCMV6-XL5) using the GenJet In Vitro Transfection Reagent for HepG2 cells (SignaGen, Rockville, MD, United States) according to the manufacturers instructions