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1 H NMR spectroscopy One-dimensional (1D) 1 H NMR spectra were recorded at 25 C on an NMR spectrometer (Bruker Ascend TM 500 NMR, Bruker Corporation, Germany) using standard NMR tubes with a diameter of 5 mm

Patients with Mitochondrial Disease: There is an increased risk of valproate-induced acute liver failure and resultant deaths in patients with hereditary neurometabolic syndromes caused by DNA mutations of the mitochondrial DNA Polymerase (POLG) gene (e.g., Alpers Huttenlocher Syndrome)