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Concerning the young age of the patient, research on the pathogenic variant in phacomatosis susceptibility genes ( NF1, NF2, SPRED1, SMARCB1, LZTR1) and other 24 genes relevant in the pathogenesis of nervous system tumors ( TP53, EGFR, PDGFRA, PTEN, PIK3CA, PIK3R, RB1, NOTCH1, CDK4, CDKN2A, CDKN2B, IDH1, IDH2, FUBP1 CIC, TACC3, TERTp, ATRX, DAXX, FGFR3, ACVR1, H3F3A, KIAA1549 BRAF) , was performed using a customized gene panel on lymphocyte DNA and tumor tissue

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Addressing these challenges will require the adoption of integrative, methodologically rigorous strategies aimed at refining current analytical paradigms, thereby enabling more robust mechanistic interpretation of gut microbiome data within the context of disease pathogenesis