Available from: 85.Butterfield JH, Weiler CR
Shell make a wonderful, easy-going house dogthe sort to be perfectly happy hanging out nearby while you relax
BSO treatment increased the transcription of these transporters (Figure 5)
Pull-Down Assay Service Modules We Offer ProteinProtein Interaction (Co-IP / APMS) Capture native or recombinant complexes using GST, His, FLAG, HA, or biotin tags Adjustable wash stringency tiers to balance specificity and yield End-to-end support from bait validation to MS-based interactor profiling Small Molecule / PROTAC / Fragment-Based Pull-Down Covalently couple compounds to solid supports via amine/NHS or alkyne/click chemistries Include competitive elution to confirm binding specificity Ideal for chemoproteomics, target deconvolution, or hit validation Use biotinylated oligos, structure-stabilized aptamers, or motifs Optional RNase/DNase protection strategies Scrambled/mutant sequence controls to verify sequence specificity Lipid or Membrane-Targeted Pull-Down Employ lipid-coated beads, liposomes, or nanodiscs Enrich lipid-binding proteins or membrane-associated complexes Compatible with detergents and membrane mimetics Crosslinking-Assisted Capture Stabilize weak or transient interactions using MS-compatible crosslinkers (e.g., DSSO, BS3, DSP) Applicable to dynamic complexes or interaction time-course studies Combine with label-free or TMT quantification for comparative analyses Quantitative Mass Spectrometry Readouts Label-Free Quantification (LFQ): For simple comparisons and pilot studies TMT/iTRAQ Multiplexing: For multi-condition, high-throughput workflows (up to 18-plex) Targeted Validation: Confirm top hits using PRM (Orbitrap) or MRM (Triple Quad) Data-Independent Acquisition (DIA): For robust, reproducible depth across samples When to Choose Each Pull-Down Modality Tagged bait available, unknown partners : Proteinprotein pull-down with tag-only and beads-only controls
10.1186/s13024-019-0341-5 65 TrenzT
