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While rats were in a restrainer, 1.5 mL saline containing phenol red (0.5 mg/mL) at the speed of 0.1 mL/min was injected into the proximal colon via the inserted catheter and the anal effluent was collected in a plastic weighting boat every 10 min for a period of 100 min for evaluation of the output percent of phenol red representing colon transit