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Serum B12 levels were measured at baseline (day 0) and on days 1, 3, 5, and 7

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Western blotting After treatment with bortezomib (4 nM), UroA (15 M), their combination, or vehicle (as a control), cells were lysed and cleared by centrifugation (14,000 g , 4 C, 5 min), and the protein concentration was determined with the Quick Start Bradford 1 Dye Reagent (Bio-Rad Laboratories, Hercules, CA, USA) on the Epoch microplate reader

CJC-1295 and ipamorelin are research peptides