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Although the mechanism of EMS-induced granulosa cell death is unknown, oxidative stress-induced apoptosis could be produced by endoplasmic reticulum stress

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The skin incisions were closed and rats were returned to their home cages after completely recovered from anesthesia on a soft heating pad

0.1% (v/v) formic acid and 2% (v/v) acetonitrile in water was used to wash the trap cartridge, followed by the separation of the concentrated peptides using a SCIEX capillary reverse-phase column (ChromeXP, 3C18-CL-120, 3 m, 120 and 0.3 150 mm)