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1 10 4 293T cells were seeded in each well of a 24-well plate overnight, following which they were co-transfected with wild-type and mutant AQP4 promoter-luciferase plasmids (0.1 g per well) and 0.4g miR-146a-3p mimics or NCs using Lipofectamine 2000 (Invitrogen, United States)

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After oral administration, the majority of curcumin is excreted as metabolites, with only a small fraction entering the bloodstream, at concentrations well below those required to inhibit most of its anti-inflammatory targets