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After 24 hours, cells were washed with saline to remove non-internalized bacteria and lysed with 0.1% saponin (Sigma-Aldrich) per 10-20 minutes at 37 C

Amino acid losses during hemodialysis with polyacrylonitrile membranes: effect of intradialytic amino acid supplementation on plasma amino acid concentrations and nutritional variables in nondiabetic patients

38,39 Sorafenib and cabozantinib almost completely depleted the pERK signal, while AXT050 and rilotumumab showed inhibition in a range of 882% and 1884%, respectively, across the patients

10.1038/s41541-020-00237-9 259 WuX.JinS.DingC.WangY.HeD.LiuY