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One half-sample was resuspended in 100 mM HEPES pH 8.5, 2% SDS, 1 mM EDTA, 1 mM DTPA, 10 M neocuproine, and 35 mM IAM for 2 hours at 37C in the dark, and the other half-sample was treated with labeling buffer 100 mM HEPES, 2% SDS, 1 mM EDTA, 1 mM DTPA, 10 M neocuproine, and 35 mM CPT