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To construct the modified homozygous strain expressing GAP1 as the counter-selectable marker in the BY4743 background, the KanMX fragment was amplified from pFA6A-KanMX6 [32], and HS fragments for pGAP1 were amplified from yeast genomic DNA and these fragments plus pGSH1 (above) assembled into pRS315 to give pRS315- HS-KanMX-pGSH1-HS

Kinetic modeling calculation The permeation of AuNPs through gaps opened in the BBB was calculated by integrating Eqs

Several types of medicines are often used to manage IBD, including aminosalicylates, steroids, immunosuppressants and biologics

doi:10.1016/j.numecd.2022.05.008 Jerneren F, Cederholm T, Refsum H, et al