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Intracellular iron is mostly stored in a nonbioavailable composition by binding to ferritin, yet free iron is required for oxidative modifications to occur during Fenton reactions

In addition, the choice of an affinity can also rely on the size: small tags are useful for protein detection and antibody production, as they are not immunogenic as large tags (Terpe, 2003)

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The free aldehyde in the open-chain form of the alkenal adduct can react with a second lysine, histidine, or cysteine and then can act as a heterobifunctional cross-linking reagent