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Silver staining Following SDS-PAGE, gels were silver stained by immersing them in 50% methanol and 10% acetic acid for 30 min, then in 5% methanol for 15 min, and finally washing three times with ultrapure water (5 min each)

Osteoporosis is often associated with high bone turnover (increases in bone resorption as well as in bone formation) which results in decreased BMD

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Iliescu C, Balanescu D, Hassan S, Lopez-Mattei J, Palaskas N , Dasari A, Halperin D, Yao J, Kar B, Gregoric I, Nathan I, Durand JB, Karimzad K, Kim P, Iliescu G, Balanescu S, Marmagkiolis K, Donisan T