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Taking a 30-day break allows receptors to reset and regain sensitivity, ensuring that the next cycle remains effective

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Proteins required identification with at least two distinct peptides and quantification in at least two technical replicates across four biological replicates, with average intensities calculated for each group

For DBS and VAMS just a simple extraction step with organic solvent mixtures is needed prior to HPLC-MS/MS analysis but, depending on the blood volume sampled, lower analytical performance has to be taken into account (Osteresch et al

Refrigeration between 2C and 8C is recommended to prevent degradation