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Ann Thorac Surg 113(3):1008-1014, 2022

We recommend using a #3 seed plate and modifying the hole size to fit the variety being planted

However, some limitations remain: First, there is inadequate resolution to completely remove co-sedimented non-vesicular elements (for example, protein aggregates or extracellular nucleic acids)

Meanwhile, the surrounding normal tissues are not directly affected by cryotherapy and may still provide relatively favorable conditions for the virus, which may lead to further viral spread (3)

However, the findings from observational studies remain inconclusive (Romagnolo And Selmin 2012)