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Tong TYN, Appleby PN, Bradbury KE, Perez-Cornago A, Travis RC, Clarke R, Key TJ

Thus, for optimization purposes, research on the ideal cell state for a given biotransformation needs to be carried out

Artificial optimized 5 untranslated regions (5 UTRs) including the ribosome binding sites (RBS) were generated for each gene and vector combination with the online RBS calculator of the Salis Laboratory of Penn State University 2 and were introduced via the oligonucleotides during cloning

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Individuals with severe MMA are at increased long-term risk for neurologic injury (e.g., metabolic stroke, seizure), poor feeding, and failure to thrive [6]