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Apple embryonic axes (25 mg were homogenised in 50 mM K-phosphate buffer pH 7.5 containing 5 mM DTT, 5% (w/v) glycerol, 0.1% (w/v) triton X-100, 0,1% (v/v) protease inhibitor cocktail and 2% (w/v) PVPP on ice

doi: 10.1038/s41467-020-20154-8

This is because many nutrients are required for proper digestion, metabolism, and excretion of various substances

[194] Common adverse effects reported in clinical studies include gastrointestinal distress, nausea, and headaches, while more serious concerns involve elevated liver enzymes, jaundice, and systemic toxicity

This shift increases pro-inflammatory cytokines, such as IL-12, and decreases immunosuppressive cytokines like IL-10, enhancing the immune response within the tumor microenvironment