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Directly determining the binding affinities of inhibitors using isothermal titration calorimetry and surface plasmon resonance is too costly

Figure 8 To determine whether H 2 O 2 contributes to cell death, cells were pre-treated with 0.1mM N-acetylcysteine (NAC), a thiol-containing antioxidant that supports intracellular redox buffering, and monitored over a 72-hour period ( Figure 8D )