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In a 2014 study, Xiao et al

Grape seed procyanidin B2 protects podocytes from high glucose-induced mitochondrial dysfunction and apoptosis via the AMPK-SIRT1-PGC-1 axis in vitro

Generation of lentiviral particles Lenti-X 293T cells were used to package the pKLV guide vectors (pKLV-PU6-gRNA-BbsI-pPGK-Zeocin-mTagBFP2) and proteins for overexpression into lentiviral particles