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Yue W, Ying Y, Wang C, Zhao Y, Dong C, Whelan J, Shou H (2017) OsNLA1, a RING-type ubiquitin ligase, maintains phosphate homeostasis in Oryza sativa via degradation of phosphate transporters

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The reaction mixture was incubated at 30 C and the consumption of NADH was monitored continuously for 60 min with a TECAN M200 plate reader by measuring the absorbance at 340 nm

The -syn-FLAG/anti-FLAG beads were washed twice with BA100 buffer and three times with LLPS buffer before being incubated with either UBQLN1, UBQLN2, or UBQLN4 recombinant proteins for 2 h at 4 C with constant rotation