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In fructose-induced diabetic rats, GA increased the expression of proteins related to hepatic insulin signalling, including insulin receptor (IR), insulin receptor substrate 1 (IRS-1), phosphoinositide 3-kinase (PI3K), Akt and glucose transporter 2 (GLUT2) (Huang et al., 2016)

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For the index PCR, a 25 L reaction containing amplicon PCR product, 0.5 L of Platinum SuperFi II DNA Polymerase, 1 SuperFi II Buffer (Thermo Fisher Scientific), and 0.5 M each of Nextera XT Index Kit v2 index primers (Illumina) was prepared

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