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Piya S, PhD, Ma H, MS, Piya S, PhD, Ruvolo V, MS, Basyal M, BSc, Golas A, PhD, Windak R, PhD, Rzymski T, PhD, Andreeff M , MD, PhD, Borthakur G, MD

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Seahorse XF RPMI medium (Agilent, #103576-100) supplemented with 2 mM Glucose (Agilent, #103577-100), 2mM Glutamine (ThermoFisher ScientificTM, #25030-024), 0.2% sodium bicarbonate (ThermoFisher ScientificTM, #25080-094) and 1% FBS was used for experiments carried out in low nutrient conditions with both cell lines

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On the other hand, the liver sections from the MTX-treated group showed several changes such as infiltration of inflammatory cells (Fig