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Add the elution buffer to the column, cap both ends, and incubate for a few minutes before uncapping and dripping your elution out of the bottom (Figure 4)

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Induction of this biosynthetic pathway enhances intracellular GSH levels and promotes cancer cell resistance to ferroptosis, 142,143 whereas pharmacological inhibition of GSH synthesis by L-buthionine-(S,R)-sulfoximine (BSO), a GCL inhibitor, induces ferroptosis 144

After 1 h incubation with secondary antibody solution, membranes were imaged using the femtoLUCENT PLUS-HRP Kit (G-Biosciences) in a Qiagen ChemiDoc TM imaging chamber

It binds to harmful substances and helps flush them out