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Adult mouse astrocyte isolation Brain tissues were aseptically removed, minced and enzymatically digested in HBSS (Gibco, 14175103) containing 50 mg ml 1 collagenase D (Sigma-Aldrich, 11088858001), 100 g ml 1 TLCK trypsin inhibitor (Sigma-Aldrich, T9378), 100 U l 1 DNase I (Sigma-Aldrich, D5025), and HEPES pH 7.2 (Gibco, 15630080) for 1 h at 23 C with shaking

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Thorax 66(6):521527

Practice of regular physical and/or mind-body mindfulness and meditative activities have been shown to modulate endocannabinoid (eCB) levels, in addition to other players as brain-derived neurotrophic factor (BDNF)