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In order to reduce biases in analysis, artifacts such as low-quality reads, adaptor sequence, contaminant DNA, or PCR duplicates were removed (Trimmomatic0.38) 32

They found that approximately half of these single cells were classified as DTCs originating from primary tumors and/or lymph node metastases, while the remaining CNA-positive cells were present in the bone marrow of non-metastatic patients

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