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Lastly, the changes associated with cell diffusion and invasion led to the interrogation of cell stiffness where it was observed that initial GPx4 overexpression increased U251 cell stiffness from 300 to 365 Pa, however, a high level of heterogeneity prevented statistical significance ( p = 0.48, Fig

The first sample was dissolved in methanol with 0.1 mL of 50 % of the SSA and the second sample in an ammonium buffer pH 3.5 [H 2 O/ACN (90:10) (v/v) + 1 mM NH 4 (CH 3 COO)] with CH 3 COOH

Conceiving of the study, participated in its design and helping to draft the manuscript

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