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Description

The results from both the cell survival fluorescence assay (Figure 4A) and the CCK-8 assay (Supplementary Figure S6A) indicated that a concentration of 1 mM had the strongest toxic effect on HK-2 cells

2.8 Measurement of nucleoside triphosphates Nucleotides were extracted from 4 to 5 10 6 cell pellets using perchloric acid, and the extracts were neutralized with potassium hydroxide