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doi:10.1016/j.bcmd.2014.06.006 [PubMed] 18

Consider your dose requirements and measurement precision when deciding

SetCys peptide 1 (1.32 mg, 0.881 mol, 1.2 equiv), cysteinyl peptide CILKEPVHGV-NH 2 (1.27 mg, 0.884 mol, 1.2 equiv) and peptide thioester 4 (1.14 mg, 0.735 mol, 1 equiv) were then successively dissolved in this solution (92 L) and the reaction mixture was stirred at 37 C