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Sections were treated with the primary rabbit anti-GSH (reduced) antibody (Agrisera) diluted 1:25 in 0.1% BSA-C in PBS (136 mM NaCl, 2.68 mM KCl, 10 mM Na 2 HPO 4 , 1.7 mM KH 2 PO 4 , pH 7.4) at 4C overnight

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Both animals received the higher doses from the beginning, and the blood sampling schedule was changed to collecting blood for up to 3 weeks and 1 week prior to vaccination, on vaccination day, and 2, 3 and 4 weeks following each injection to better resolve the vaccine responses over time

Longhi, editor, Managing psychosexual consequences in chronic diseases (pp