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Extract analysis by high-performance liquid chromatography The constituents of the bilberry and ginger root extracts were analyzed by reversed phase-HPLC (RP-HPLC) using an Agilent 1,200 Series HPLC System equipped with a ZORBAX Extended-C18 column (2.6 mm ~ 250 mm, 5 m) and a diode array detector (G1315D) (Agilent Technologies Inc., Mississauga, ON, Canada)