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Cells were deprived of growth factors by incubation in starvation media RPMI-1640 containing 0.5% (w/v) BSA (Sigma, St Louis, MO, USA) for 18 h before stimulation experiment with HGF (40 ng ml 1 for 7.5 min)

These include: Broccoli Cauliflower Cabbage Garlic Onions Brussels sprouts Eggs Lean proteins Vitamin C-rich foods such as oranges, kiwi, guava, and strawberries may also support glutathione activity

Rough estimations point to around 500,000 IMD cases worldwide, causing around 50,000 deaths each year (World Health Organization, 2025)

Proper hygiene is maintained