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Many people will also experience cognitive difficulties such as problems with vision, memory, thinking, or reasoning, the development of mental illnesses such as depression, or general changes in behavior

Patient safety advice: Never stop prescribed B12 treatment without consulting your doctor, even if levels are elevated

Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark

10.1128/aem.02706-19 113 WillemsR