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doi: 10.1210/me.2011-1379 54 AielloAPandiniGSarfsteinRWernerHManfiolettiGVigneriRet al

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Sackesen C, Ercan H, Dizdar E, Soyer O, Gumus P, et al: A comprehensive evaluation of the enzymatic and nonenzymatic antioxidant systems in childhood asthma

That variation is chemically real and it is small: it moves the copper mass fraction by less than a tenth of a percentage point, which is far inside the uncertainty introduced by salt form and residual water in an actual vial

Subcutaneous tissue and muscles were separated by blunt dissection with forceps, the dura mater was blotted dry with a sterile cotton swab, and CSF was collected using a sterile pulled capillary to penetrate the cisterna magna (through the dura mater, lateral to the arteria dorsalis spinalis) and immediately snap-frozen in liquid nitrogen and stored at 80 C