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Chromatin immunoprecipitation (ChIP) Cells were synchronized at the G1/S phase using a double-thymidine block

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It is therefore not surprising that the olfactory system has one of the highest MT abundances of the mammalian central nervous system, and that expression of the MT-1, -2, and especially -3 isoforms are greatest in the olfactory neurons of the brain and the anatomically adjacent hippocampal region, which has direct nerve fiber projection contacts from the olfactory bulb [94, 101, 103105]

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The LR3 modification adds a 13-amino-acid extension and replaces one amino acid, which reduces binding to the proteins that normally deactivate IGF-1 in your bloodstream