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Quick Guide: Preparing for AOD-9604 Therapy Proper preparation for AOD-9604 involves medical evaluation, lifestyle adjustments, and understanding administration protocols: Complete baseline bloodwork including metabolic panel, thyroid function, and hormone levels Consult with a qualified physician to confirm AOD-9604 is appropriate for your health profile Establish consistent sleep patterns of 7-8 hours nightly for optimal hormone function Begin a protein-forward, moderate-calorie diet 1-2 weeks before starting therapy Plan for morning fasted administration to maximize fat oxidation Reduce alcohol consumption and eliminate it 48 hours before your first dose Prepare proper storage (refrigeration) and sterile injection supplies Set realistic expectations for gradual results over 8-12 weeks Your preparation phase is just as important as the therapy itself

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Cytokine and chemokine assays Protein was extracted using a 2-cm piece of distal colon and homogenized in 300 l of Meso Scale Discovery lysis buffer with protease and phosphatase inhibitors as described in the assay protocol