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Conformational and oligomeric effects on the cysteine pK(a) of tryparedoxin peroxidase

CYP2E1 is induced after chronic ethanol ingestion and because is a poorly coupled enzyme formation of ROS arises even without substrate

Bound proteins were eluted with His elution buffer (50 mM Tris-HCl pH 7.5, 50 mM NaCl, 250 mM imidazole, 2.5 mM ME)