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In this work, we used a combination of biophysical techniques and cryo-electron microscopy (Cryo-EM), showing that human CBS full-length protein polymerises as a filament adopting two very different morphologies dependent on SAM binding and that polymerisation plays a role in both enzyme activation and stability

Ichim TE, Minev B, Braciak T, Luna B, Hunninghake R, Mikirova NA, Jackson JA, et al

The samples were centrifuged at 15,000 rpm for 2 min at 4 C using an Eppendorf 5424R centrifuge (Eppendorf SE, Hamburg, Germany), and the resulting supernatant was subjected to HPLC-MS/MS analysis

Gamma-aminobutyric acid (GABA) GABA is an inhibitory neurotransmitter, turning down the rate of electrical impulses in the brain.81 Antiepileptic medications, like Lamictal and valproic acid, raise GABA levels

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