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However, high protein yields cannot be achieved with the Flag-tag due to low capacity

2023 Lee et al

Weight gain that occurs early (before 20-24 weeks) seems to be crucial

Cell Factories 2023, 22, 224

In the mouse heart, the abundance of AC5 protein reaches its highest point before 1 week and then decreases to levels similar to those of mice studied at 3, 6, and 24 months of age