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Notably, CRE sequences vary among genes, often exhibiting one or two base substitutions and even half-CRE sites (such as TGACG or CGTCA) can be recognized by ATFs, suggesting that while the core CRE motif is evolutionarily conserved, a certain degree of sequence flexibility is tolerated (103)

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To reduce the urea concentration, the samples were diluted 5-fold with ammonium bicarbonate (AMBIC) before overnight digestion at 37 C with trypsin (type IX-S from Porcine Pancreas) using a 1:30 (w/w) enzyme to substrate ratio

10.1016/S1470-2045(22)00372-2 150 LoughreyD.DahlmanJ