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1 and Table 1 ) using Recon 2.04 as input reconstruction, RPMI composition as medium constraint and the biomass function and ATP maintenance as objective functions

The prognosis for unresectable or metastatic CCA remains dismal, with median survival limited to approximately 1 year [5]

coli and purified using IPTG (Roth), Ni-NTA Agarose (Invitrogen, Karlsruhe) and Imidazole (Roth, Karlsruhe) (78) and native human TXNRD1 was isolated from human placenta (32)