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SchranteeATammingaHGBouzianeCBottelierMABronEEMutsaertsH-JMet alAge-dependent effects of methylphenidate on the human dopaminergic system in young vs adult patients with attention-deficit/hyperactivity disorder: a randomized clinical trial

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Briefly, the anti-MBP HC and LC lacking native export signals were PCR-amplified from pNEB-LC-MBP and pUC57-HC-MBPsyn, respectively, and subsequently assembled as a bicistronic operon using overlap extension PCR

The removal of the staining working solution and subsequent rinsing of the cells with PBS was imperative, pre-warmed PBS was added and the cells were incubated for 5 min and photographed and documented under an inverted fluorescence microscope