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Quantification of 2HG enantiomers 2HG quantification in SCD erythroblasts was performed as we previously described 24 by liquid chromatographymass spectrometry (LC-MS/MS) after derivatization using N-(p-Toluenesulfonyl)-L-phenylalanyl Chloride (TSPC) on the LC-ESI-MS/MS system

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Single-cell analysis of mouse gastrulation and early organogenesis 19 revealed remarkably high Gpx2 expression in DE and gut at embryonic days (E) E6.5-8.5 (Fig