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The isolated nuclear Protein was used for western blot analysis with the Histone H3 antibody (Abmart, P30266M).The supernatant was subjected to ultracentrifugation at 100000 g for 3 h, and the resulting supernatant was collected as the cytoplasmic protein fraction.The isolated cytoplasmic protein was used for western blot analysis with the Actin antibody (Abmart, T40104S).Chloroplast protein isolation: Rice leaves was soaked in 0.5% sarkosyl solution for 3-5 min, rinsed thoroughly, and ground using a mortar and pestle in 1.25 M NaCl, 40 mM HEPES (pH 7.6), 5 mM EDTA (pH8), 0.1% bovine serum albumin, 0.1% -mercaptoethanol following the high salt protocol

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Helps achieve even skin tone

Antibody for IL-6 (ab6672) was obtained from Abcam (Cambridge, United Kingdom) and diluted to 1:4000

doi: 10.1172/JCI200318797 113