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Nitta et al

Biomarkers of aging

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FIGURE 2 Cell Treatment and H 2 O 2 Detection To observe the difference of H 2 O 2 secretion from the single cells and cell spheroids, ascorbic acid (AA) (A17759, Alfa Aesar) is used to treat the cells ( 2 O 2 after the treatment in a time-lapse manner, 60 l of the cell suspension is collected and pipetted onto the hydrogel substrate right after (t = 0 s) and every 30 s for 4 min after the AA addition