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It should be noted that, in order to avoid any possible fluorescence from a plastic or glass support during analysis with near-UV excitation, cells were grown on quartz plates that were then placed on the microscope stage in 50-mm thermostated Petri dishes, filled with 5 mL of phosphate buffered saline (PBS)

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Demeestere I, Gervy C, Centner J, Devreker F, Englert Y, Delbaere A: Effect of insulin-like growth factor-I during preantral follicular culture on steroidogenesis, in vitro oocyte maturation, and embryo development in mice