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Description

Glutamine synthetase is an enzyme in astrocytes that breaks down glutamate into glutamine after uptake through the EAATs

Mol Pharmacol 2001

doi: 10.1016/j.jid.2021.09.015 234 JinRZhouMLinFXuWXuA

Electron microscopy sample preparation and data acquisition A 3Ll volume of 5LmgLml 1 purified SLC33A1 MAP was mixed with Pmab1 Fv-clasp at a 1:1.5 molar ratio and 20mM GSSG, incubated for 30 min on ice and then applied to glow-discharged Au 400 mesh QUANTIFOIL R1.2/1.3 holey carbon grids (Quantifoil) or Graphene Oxide Au 400 mesh QUANTIFOIL R1.2/1.3 grids before being plunged into liquid-nitrogen-cooled liquid ethane using an FEI Vitrobot Mark IV (FEI Thermo Fisher)