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Among the various ROS, H 2 O 2 triggers the redox mechanism by targeting the receptor including protein tyrosine kinases, tyrosine phosphatases, and transcription factors via secondary messenger in intercellular signaling

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Immunoblotting GAPDH Methanol precipitated protein pellets were dissolved in 50% TFE/ABC

*Correspondence: Ismail Laher, Department of Pharmacology and Therapeutics, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada V6T 1Z3

Thus, residues Tyr110 and Lys105 might share a function in accordance with the glutathione activator model but might also differ, for example, regarding the protonation state of the active site cysteine residue