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Membrane Skeletal Protein S-Glutathionylation in Human Red Blood Cells as Index of Oxidative Stress

Determination of glutathione concentrations Cells were lysed with 1% (w/v) of sulfosalicylic acid, centrifuged at 13,000 g for 5 min at 4 C, and the supernatants were used for the determination of total glutathione (that is, reduced glutathione plus twice the concentration of oxidized glutathione), using oxidized glutathione (050 M) as standard as described previously 50

4d), GA is coordinated by the active site Mg 2+ and several hydrogen bonds with residues Ser91, Gly92, Trp193, and Arg228

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