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Transwell assay The chemotactic motility of HUVECs was determined using transwell migration chambers (Corning) with 6.5 mm diameter polycarbonate filters (8 m pore size), as described previously.28 In brief, the bottom chambers were filled with 750 mL of RPMI 1640 medium containing all supplements

The purpose of this study was to illustrate how challenging it is to design a peptide-based cancer vaccine and to balance delivery method, half-life, epitope selection, and immunogenicity to produce a successful vaccine strategy

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(2019) 25:273289.e5