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First, the nutrient formulations play a significant role

Lin H-L, Chen C-S, Fink JB, Lee G-H, Huang C-W, Chen J-C, et al

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For homogenates made with glutathione, 10 mM GSH (Figure 3B, top) was able to elute the GFP completely while 10 mM cysteine (Figure 3B, bottom) was unable to elute the protein